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81.
82.
Chie SUZUKI Yosuke SAKAGUCHI Hiroyoshi HOSHI Koji YOSHIOKA 《The Journal of reproduction and development》2016,62(1):79-86
The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced
in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium
(PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates
of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured
with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of
LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with
other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and
Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured
with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes
involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3,
CPT1, CPT2 and KAT) in Day-7 blastocysts were
significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching
ability and quality of porcine blastocysts produced in vitro, as determined by ATP content,
blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of
LR-BSA arise from lipids bound to albumin. 相似文献
83.
The cultivation experiment was carried out to investigate the effects of different proportions of peat soil, perlite, vermiculite and yellow mud on growth of Gesneriaceae species(Chirita gueilinensis, Sinningia speciosa, Lysionotus pauciflorus, Hemiboea henryi, Aeschynanthus acuminatus, Saintpaulia ionantha). The growth traits of each plant growing in 7 different matrix materials were investigated.The plant height, crown width and chlorophyll content of each plant were measured. The results showed that the best substrate ratio was peat soil∶vermiculite=2∶1for C. gueilinensis, L. pauciflorus and H. henryi; peat soil∶perlite∶vermiculite = 2∶1∶1for S. ionantha; peat soil∶vermiculite∶yellow mud=2∶1∶1 for S. speciosa; peat soil∶perlite∶vermiculite∶yellow mud=2∶1∶1∶1 for A. acuminatus. 相似文献
84.
不同春小麦品种耐低磷性评价及种质筛选 总被引:2,自引:0,他引:2
筛选磷高效作物是充分利用土壤磷素和减少磷肥施用量的重要手段。本研究以162份春小麦种质资源为材料,对其苗期的株高、总根长、根表面积等8个指标的耐低磷系数进行分析,采用隶属函数法综合评价春小麦苗期的耐低磷特性,初步筛选耐低磷材料,并进一步进行成株期的耐低磷特性鉴定,筛选出耐低磷材料和磷敏感材料,分析其在低磷下酸性磷酸酶的活性变化。结果表明,低磷胁迫下春小麦材料苗期和成株期的各性状均受到不同程度的影响,并随着胁迫程度的增加,小麦生长受抑制程度增强。通过主成分分析将苗期8个指标转化成4个综合指标(累计贡献率为82.60%),将成株期的10个指标转化为3个综合指标(累计贡献率为83.23%);采用隶属函数法计算耐低磷综合评价值(D)值,对D值进行聚类分析,将苗期的162份春小麦种质资源划分为耐低磷型(10份)、较耐低磷型(26份)、低磷较敏感型(91份)、低磷敏感型(35份)4类。选取耐低磷型材料(5份)和低磷敏感型材料(4份),进一步进行成株期鉴定,最终筛选1份耐低磷材料wp-35和1份磷敏感材料wp-119。通过分析其酸性磷酸酶活性,发现在低磷胁迫下春小麦根系和叶片中的酸性磷酸酶活性均升高,且耐低磷材料的酸性磷酸酶活性高于磷敏感材料。本研究结果可为解析春小麦耐低磷特性、培育耐低磷品种提供种质资源和理论依据。 相似文献
85.
86.
研究柠条锦鸡儿(Caragana Microphylla Lam.)林地不同土层深度土壤有效磷的变化规律,为进一步深入研究北方农牧交错带土壤性质及地区植被建设提供依据。笔者通过野外调查和数据处理,对赤峰市敖汉旗风沙土区不同林龄(5、11、17、23、29年)柠条锦鸡儿不同土层的土壤有效磷进行了分析。研究表明:相同林龄不同立地条件的土壤有效磷含量随土层厚度的增加呈现减小的趋势;相同林龄不同立地条件的土壤有效磷含量大小表现为丘间低地阴坡阳坡;相同立地条件不同林龄的土壤有效磷含量随林龄的增加而增加。柠条锦鸡儿的种植有利于土壤性质向良性方向发展。 相似文献
87.
A calibration method of detecting soil water content based on the information-sharing in wireless sensor network 总被引:2,自引:0,他引:2
Rong-biao ZhangJing-jing Guo Lei ZhangYe-cheng Zhang Li-hong WangQi Wang 《Computers and Electronics in Agriculture》2011,76(2):161-168
In agricultural production, there is contradiction between the cost and accuracy of detection during the course of acquiring soil water content (θ) online. This conflict is one of the core issues of automatic water-saving irrigation technology in agriculture. At the same time, capacitive soil moisture sensor (CSMS) has received considerable attention, for it can acquire θ with low cost and high precision, and meet the application requirements of wireless sensor network (WSN). But CSMS is vulnerable to the soil temperature (Ts) and salinity (Ss) in the measurement process. Therefore, this study took EC-5 sensor for example to establish water detection calibration models of soil temperature and salinity for single sensor, using Least Squares Support Vector Machines on MatLAB (LS-SVMlab) as the tool. On this basis, we explored the spatial variability of Ts and Ss, and then a method, which could be used to calibrate the output signals of sensors in multi-point network, was proposed based on the information-sharing (Ts or Ss) technology of WSN. Through laboratory experiment, we effectively reduced the impact of soil temperature and salinity on the single sensor. In example analysis, we investigated the detection precision and costs under different information-sharing radiuses (r). And the results indicated that the method we proposed based on the information-sharing technology of WSN could successfully calibrate the influence of soil temperature and salinity on sensors in multi-point network, and it was an efficacious approach to determine the balance between the calibration accuracy of moisture sensor and the investment of agricultural production. For example, while the calibration precision of soil temperature and salinity is respectively 1%, the costs can be reduced by 30%. 相似文献
88.
M. Rokebul Anower M. D. Peel I. W. Mott Y. Wu 《Journal of Agronomy and Crop Science》2017,203(6):506-518
We previously reported an alfalfa half‐sib family, HS‐B, with improved salt tolerance, compared to parental plants, P‐B. In this study, we conducted additional experiments to address potential physiological mechanisms that may contribute to salt tolerance in HS‐B. Vegetatively propagated HS‐B and P‐B plants were treated with a nutrient solution (control) or a nutrient solution containing NaCl (EC = 12 dS/m). Shoots and roots were harvested at various time points after treatment for quantification of proline, soluble sugar, and H2O2 using spectrophotometers. Subcellular localization and quantification of Na in roots were conducted using a Na+‐specific dye under a confocal microscope. HS‐B produced 86 and 89% greater shoot and root dry biomass, respectively, compared to parental plants, P‐B, under salinity in the greenhouse. Under saline conditions the HS‐B shoots accumulated 115% and roots 55% more soluble sugars than P‐B counterparts. The non‐saline HS‐B shoots, however, showed 72% less soluble sugars than the non‐saline P‐B plants. Under saline conditions HS‐B accumulated 39% less proline in shoots, while roots accumulated 56% more proline, compared to their P‐B parents. HS‐B plants also showed a greater reduction of stomatal conductance under mild saline stress. HS‐B shoots and roots contained 3–4 times less reactive oxygen species (H2O2) after salt treatment compared to P‐B plants. Sodium localization and distribution analysis using Na+‐specific dye revealed HS‐B plants accumulated 88% and 48% less Na+ in stele and xylem vessels compared to P‐B. The study provides insights into the potential mechanisms that may contribute to salt tolerance in HS‐B: maintaining RWC by accumulating soluble sugars while reducing transpiration, maintaining healthy status by reducing oxidative stresses, and preventing salt toxicity by reducing accumulation of Na+ inside root cells and xylem. 相似文献
89.
为了找寻冰雹天气发生的原因及对农业的影响,利用多普勒雷达的反射率因子、径向速度、垂直累积液态水含量(VIL)等产品,结合高低空环流形势及地面资料,对发生在长治市2016 年6 月13 日的强冰雹天气过程进行分析。结果表明:(1)此次冰雹天气是由高空冷槽和与地面暖低压共同作用结果;(2)强回波与三体散射(TBSS)对提前预报强冰雹有较好的指示意义;速度场的辐合辐散可以判断回波未来发展趋势,增强预判准确性;当TBSS出现一定的持续性时,TBSS可以作为强冰雹预警的辅助指标;VIL是判别冰雹的有效工具之一,且对冰雹的预报有较好的指示作用;(3)加强冰雹监测,通过及时的“点”监测,实现“面”预警,是减少冰雹对农业生产造成灾害的一种有效的补充手段。 相似文献
90.